A wildlife refuge, also known as a wildlife sanctuary, is a naturally occurring sanctuary, such as an island, that provides protection for species from hunting, predation, competition or poaching; it is a protected area, a geographic territory within which wildlife is protected. Refuges can preserve animals that are endangered.
Such wildlife refuges are generally officially designated territories. They are created by government legislation, publicly or privately owned. Unofficial sanctuaries can also occur as a result of human accidents; the Chernobyl Exclusion Zone has in practice become a wildlife refuge since very few people live in the area. Wildlife has flourished in the Zone since the Chernobyl nuclear accident in 1986. 
In the United States, the U.S. Fish and Wildlife Service applies the term "refuge" to various categories of areas administered by the Secretary of the Interior for the conservation of fish and wildlife. The Refuge System includes areas administered for the protection and conservation of fish and wildlife that are threatened with extinction, as well as wildlife ranges, game ranges, wildlife management areas, and waterfowl production areas. 
In the 3rd century BC, King Devanampiya Tissa declared the area around Mihintale, Sri Lanka as a sanctuary for wildlife,  probably the first of its kind in the ancient world. According to stone inscriptions found in the vicinity, the king commanded the people not to harm animals or destroy trees within the area.
The first North American wildlife refuge, Lake Merritt Wildlife Refuge at Lake Merritt, was established by Samuel Merritt and enacted in California state law in 1870 as the first government owned refuge.  The first federally owned refuge in the United States is Pelican Island National Wildlife Refuge and was established by Theodore Roosevelt in 1903 as part of his Square Deal campaign to improve the country. At the time, setting aside land for wildlife was not a constitutional right of the president. More recently, a bi-partisan group of US House of Representatives members established the Congressional Wildlife Refuge Caucus to further the needs of the National Wildlife Refuge System in the US Congress.
Today there are several national and international organizations that have taken the responsibility of supervising numerous systems of non-profit animal sanctuaries and refuges in order to provide a general system for sanctuaries to follow. Among them, the American Sanctuary Association monitors and aids in various facilities to care for exotic wildlife. Their accredited facilities follow high standards and a rigid application processes to ensure that the animals under their care are avidly cared for and maintained.
The wildlife refuge in Brazil has as its objective protecting natural environments where conditions are assured for the existence and reproduction of species or communities of the local flora and the resident or migratory fauna. It can be constituted by private areas, as long as it is possible to make compatible the objectives of the unit with the utilization of the land of the natural resources of the location by the landowners. Public visitation is subject to the conditions and restrictions established by the Management Plan of the unit and to the regulations established by the body responsible for its administration and scientific research depends on previous authorization from the body responsible for the administration of the unit and is subject to the conditions and restrictions established by this body. 
- Crown Game Preserve — designation of wildlife refuges in Ontario, Canada
- La Senda Verde, a wildlife refuge in Coroico, Bolivia
- Elephant sanctuary (disambiguation)
- Fossil park
- National park
- National Wildlife Refuge Association
- Nature reserve
- Mulvey, Stephen (20 April 2006). "Wildlife defies Chernobyl radiation". BBC News. Archived from the original on 5 November 2017. Retrieved 13 December 2017.
- Division of Refuge Planning (National Wildlife Refuge System, Mountain-Prairie Region, U.S. Fish and Wildlife Services). "Glossary - Refuge Planning - Fish and Wildlife Service, Region 6". www.fws.gov. Archived from the original on 11 April 2017. Retrieved 10 April 2017.
- LankaLibrary (11 June 2006). "Mihintale - The first sanctuary in the world". LankaLibrary Forum. Archived from the original on 7 June 2011. Retrieved 10 August 2011.
- "Lake Merritt - Wildlife Sanctuary". City of Oakland. Archived from the original on 26 April 2012. Retrieved 22 December 2011.
- Brazil 2000. Federal Law Nº 9.985 of 07/18/2000. Regulates article 225 of the Federal Constitution and institutes the National System of Units of Conservation and other provisions. Archived 30 March 2013 at the Wayback Machine.