The Washington Female Seminary was a Presbyterian seminary for women operating from 1836 to 1948 in Washington, Pennsylvania. During the 19th century, it was "one of the best known and most noted institutions of its kind in the state". 
The Washington Female Seminary was part of a larger Female seminary movement. Creation of this particular institution began in 1835 and the Seminary opened 1 year later in 1836.  The two founders were abolitionist Francis Julius LeMoyne and Alexander Reed. 
It was formally chartered by the legislature in 1839.  The first principal was Mrs. Francis Biddle of Philadelphia, who left in 1840.  Sarah B. Hanna (a student of Emma Willard) was Principal from 1840-1874.  Miss Nancy Sherrard followed as Principal. 
By 1886, attendance hovered around 100 to 150 students.  The curriculum included both a preparatory course, which generally gained admission to the finest women's colleges, and a regular course, with studies in music, art, and elocution.  It was one of the few schools that taught the Bible from a literary point of view. 
Rebecca Harding Davis, who graduated in 1848, is its most famous graduate.  In April 2013, a historical marker in Davis' honor was placed near Swanson Science Center, the site of the former McIlvaine Hall/Washington Female Seminary.  The effort to place the marker there was led by Washington & Jefferson College English professor, Dr. Jennifer Harding. 
- Creigh, Alfred (1871). History of Washington County. B. Singerly.
- Wickersham, James Pyle (1886). A History of Education in Pennsylvania, Private and Public, Elementary and Higher: From the Time the Swedes Settled on the Delaware to the Present Day. Inquirer Publishing Company. p. 490.
- Maguire, J. Simonson (1910). "The First Washington". The National Magazine. 32. Boston: Chapple Publishing Company. pp. 706–711.
- "PHMC Historical Markers" (Database search). Pennsylvania Historical and Museum Commission. Retrieved May 30, 2013.
- Mansfield, Katherine (June 30, 2013). "Descendant secures state marker for Rebecca Harding Davis". Observer-Reporter. Archived from the original on May 30, 2013. Retrieved May 30, 2013.
- Text on the John L. Stewart Tower at the former McIlvane Hall at Washington & Jefferson College
- "Ralph Cooper Hutchison (1931-1945)". U. Grant Miller Library Digital Archives. Washington & Jefferson College. 2003-09-04.
Media related to Washington Female Seminary at Wikimedia Commons