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The Jefferson Memorial Forest is a forest located in southwest Louisville, Kentucky, in the Knobs region of Kentucky. At 6,500 acres (26 km2), it is the largest municipal urban forest in the United States. 
The forest offers over 35 miles (50 km) of various hiking trails, including several which offer views of downtown Louisville. Several discrete usage areas are featured, including the Tom Wallace Recreation Area, with the 7-acre (28,000 m2) Tom Wallace Lake; the Paul Yost Recreation Area, and the Horine Conference Center. Camping and fishing are both permitted. Tom Wallace Lake is stocked with trout and catfish once a year. Tom Wallace Recreation Area features various handicapped-accessible facilities, including a fishing dock and a 1,560-foot (480 m)-long natural trail, the Tuliptree Trail. The Horine Conference Center is a popular field trip destination for Louisville schools.
The forest property is operated as parkland by Louisville Metro Government.
A hiking trail, the Siltstone Trail, traverses much of the forest from east to west. There are several local hiking trails, in addition. Horine also features many hiking trails and both the Paul Yost and Tom Wallace Recreation Areas have horse trails. No mountain biking is permitted in the forest at this time, but the low traffic roads and hilly terrain afford road cyclists many challenging routes through the forest and surrounding areas.
In 1946, Jefferson County, Kentucky, undertook to establish a working forest preserve in the southern part of the county. The Jefferson County Memorial Forest was originally envisioned to be 10,000 acres (40 km2) and was named as a memorial to the area's dead of World War II. Since then, the forest has been redesignated to remember all who served in the armed forces. The original purchases were guided by Paul Yost, who was appointed as the county forester. Through 1954, some 1,300 acres (5.3 km2) were purchased. No further properties were purchased until a single tract was acquired in 1965. The next acquisition was not until 1979, and from then until the mid-1980s, the forest was expanded to about 5,000 acres (20 km2). Since then, acquisition has proceeded again slowly.
In the late 1990s, the old ranger station, a former country schoolhouse, was renovated as a visitor and welcome center.
On May 30, 2004 parts of the park were ravaged by a tornado, which caused several trails to be temporarily closed.
There are some fifty types of trees, including ten species of oaks, and a rich flora of wildflowers and seventeen species of ferns and fern allies. A wide variety of animals can be seen, including bobcats, coyotes, red foxes, white-tailed deer, great blue herons and horned owls.
Like many other natural areas in the eastern United States, the forest has a significant problem with invasive exotics, including tree-of-heaven (Ailanthus altissima), autumn olive (Eleagnus umbellata), Amur honeysuckle (Lonicera maackii), Japanese honeysuckle (Lonicera japonica), and princess tree (Paulownia tomentosa).
The forest is located in the Knobs region of Kentucky, also known as the Muldraugh Escarpment. This is a belt of rugged hills lying between the Bluegrass and the Pennyrile regions. The underlying geology of these hills is primarily siltstone and shale, with the siltstone creating extremely steep hillsides. The most important of these in the forest area is the Holtzclaw Siltstone, named after Holsclaw Hill.
- The Parklands of Floyds Fork
- List of parks in the Louisville metropolitan area
- List of attractions and events in the Louisville metropolitan area
- City of Parks
- Valley of the Drums, a toxic waste dump near Jefferson Memorial Forest