I-81 highlighted in red
|Maintained by TDOT|
|Length||75.66 mi  (121.76 km)|
|South end||I-40 near Dandridge|
|North end||I-81 at Bristol|
|Counties||Jefferson, Hamblen, Greene, Washington, Sullivan|
Interstate 81 (I-81) is an 855-mile (1,376 km) long expressway stretching from Dandridge, Tennessee northward to the Thousand Islands Bridge at the Canada–US border near Fishers Landing, New York. In Tennessee, I-81 travels 75.66 miles (121.76 km) from its southern terminus at I-40 in Dandridge to the Virginia state line in Bristol.
Interstate 81 begins in Tennessee at Interstate 40 in Dandridge. I-81 parallels the Appalachian Mountains for most of Tennessee and Virginia. At mile marker 8, I-81 meets U.S. Route 25E south of Morristown. I-81 doesn't go through either Johnson City or Kingsport; but at mile marker 57, I-81 meets Interstate 26 and U.S. Route 23 which go to Kingsport and Johnson City. At mile marker 75, I-81 leaves Tennessee and enters Virginia.
|Interstate 181||Johnson City and Kingsport||Former spur||Was renumbered as Interstate 26 by March, 2007|
Construction on I-81 in Tennessee began in the 1960s.  The contracts for most sections of the route were awarded in 1969 and 1970.  By December 1974, the entirety of the route was open to traffic, and most was completed.  The final section in Tennessee was completed in August 1975. 
|Jefferson||Dandridge||0.00||0.00||1||I-40 – Asheville, Knoxville||Southern terminus; I-40 exit 421; signed as left exit 1A (east) & 1B (west).|
|White Pine||4.4||7.1||4||SR 341 (White Pine Road) – White Pine|
|Hamblen||Morristown||8.6||13.8||8||US 25E – White Pine, Morristown|
|12.4||20.0||12||SR 160 – Lowland, Morristown|
|||15.3||24.6||15||SR 340 (Fish Hatchery Road)|
|Greene||Mosheim||23.1||37.2||23||US 11E – Mosheim, Greeneville, Bulls Gap||Access to Tusculum College|
|||30.2||48.6||30||SR 70 to SR 66 – Rogersville, Greeneville|
|Baileyton||36.2||58.3||36||SR 172 (Baileyton Road) – Baileyton, Greeneville|
|Washington||Fall Branch||50.5||81.3||50||SR 93 to SR 81 – Fall Branch, Jonesborough|
|57.7||92.9||57||I-26 / US 23 – Johnson City, Kingsport||Signed as Exits 57A (east) & 57B (west), I-26 exits 8A-B; former I-181 south|
|59.4||95.6||59||SR 36 (Fort Henry Drive) – Johnson City, Kingsport, Colonial Heights|
|63.5||102.2||63||SR 357 south (Airport Parkway) to SR 75 – Tri-Cities Regional Airport||Also access to Northeast State Community College|
|66.1||106.4||66||SR 126 (Memorial Boulevard) – Blountville, Kingsport|
|Blountville||69.6||112.0||69||SR 394 – Blountville, Bristol||Access to Bristol Motor Speedway|
|Bristol||74.8||120.4||74||US 11W (West State Street) – Kingsport, Bristol||Signed as Exits 74A (north) & 74B (south)|
|75.66||121.76||I-81 north – Roanoke||Virginia state line|
|1.000 mi = 1.609 km; 1.000 km = 0.621 mi|
- 2007 Rand McNally Atlas
- "Route Log - Main Routes of the Eisenhower National System Of Interstate and Defense Highways - Table 1". Federal Highway Administration. Retrieved 4 October 2014.
- "Where Do We Stand On The Interstate?". Kingsport Times-News. May 2, 1965.
- "81 Moving Along:Chamber". Kingsport Times-News. May 2, 1965. Retrieved 2018-06-18.
- "East Tennessee's Christmas Present". Kingsport Times-News. December 10, 1974. Retrieved 2018-06-18.
- "Interstate 81 Four-Lane Opens". Kingsport Times-News. August 28, 1975.
- Media related to Interstate 81 in Tennessee at Wikimedia Commons