Despite producing a large amount of seeds per seed pod, it reproduces largely by vegetative reproduction,  and remains restricted to the North East region of the United States and south east regions of Canada. Although never common, this rare plant has vanished from much of its historical range due to habitat loss.  It has been a subject of horticultural interest for many years with Charles Darwin who, like many, was unsuccessful in cultivating the plant. 
The species name reginae is Latin for "of a queen". Common names also include fairy queen, white wing moccasin, royal lady's slipper, nervine, and silver-slipper. 
The plant became the state flower of Minnesota in 1902 and was protected by state law in 1925. It is illegal to pick or uproot a showy lady's slipper flower in Minnesota.
Although this plant was chosen as the provincial flower for Prince Edward Island in 1947, it is so rare on the island that another lady's-slipper, C. acaule (moccasin flower or pink lady's slipper), replaced it as the province's floral emblem in 1965.  
Cypripedium reginae grows in wetlands such as fens and open wooded swamps that are sometimes populated by tamarack and black spruce. Cyp. reginae thrives in neutral to basic soils but can be found in slightly acidic conditions. The plants often form in clumps by branching of the underground rhizomes. Its roots are typically within a few inches of the top of the soil. It prefers very loose soils and when growing in fens it will most often be found in mossy hummocks.
This photo, taken in a forested, calcareous fen in Williamstown, MA, is only one of 14 occurrences currently documented in the state (1984 to the present 2016).  The increasing rarity of this plant is attributable to destruction of a suitable alkaline habitat – and an exploding deer population whose browsing stunts or eliminates its growth. It can tolerate full sun but prefers partial shade for some part of the day. When exposed to full sun, the flower lip is somewhat bleached and less deeply colored. It is occasionally eaten by white-tailed deer. Cypripedium reginae can be found in Canada from Saskatchewan east to Atlantic Canada, and the eastern United States south to Arkansas and Tennessee. 
Cypripedium reginae is quite rare, and is considered imperiled ( SRANK S2) or critically imperiled (S1) in Arkansas, Connecticut, Illinois, Iowa, Missouri, New Brunswick, New Hampshire, New Jersey, Newfoundland and Labrador, North Dakota, Nova Scotia, Ohio, Pennsylvania, Prince Edward Island, Saskatchewan, Tennessee, Virginia and West Virginia. Additionally, it is considered vulnerable (S3) in Indiana, Maine, Manitoba, Massachusetts, New York, Quebec, Vermont, Minnesota, Wisconsin, Rhode Island, and several areas of east Canada. 
The showy lady's-slipper is sensitive to hydrologic disturbances, and is threatened by wetland draining, habitat destruction and horticultural collectors.
The showy lady's slipper is a popular plant among orchid collectors for its color and structure. However, it has proven to be a difficult plant to cultivate, due to its poor seed germination and slow maturation to flowering. This makes it more vulnerable to illegal collection. It was difficult to raise from seed, taking many months to germinate in sterile culture until progress on axenic culture from seed in the 1990s by a group of high school students in New Hampshire.     Efforts at micropropagation have had marginal success. 
Cyp. reginae reproduces sexually and depends on insects such as syphid flies, beetles and Megachile bees for pollination. The structure of the flower creates a tight space through which insects have to squeeze. A pollinating insect first passes by the stigma, and upon exiting the trap rubs against the anther. Pollination typically occurs in June and the seed pod or fruit is ripe by September and dehisces by October.  Although a single seed pod can produce over 50,000 seeds, low germination and a seed-to-flowering term of about 8 years indicate that sexual reproduction is inefficient. Asexual reproduction from rhizomes in the Showy Lady's slipper is a common means of sustaining a population.
It flowers in early to midsummer, usually with 1 to 2 flowers per stalk, less commonly 3 or 4.
Cypripedium reginae contains an irritant, phenanthrene quinone or cypripedin. The plant is known to cause dermatitis on the hands and face. The first report of the allergy reaction was first reported in 1875 by H. H. Babcock in the United States, 35 years before the term " allergy" was coined. The allergen was later isolated in West Germany by Bjorn M. Hausen and associates. 
The Cypripedium species have been used in native remedies for dermatitis, tooth aches, anxiety, headaches, as an antispasmodic, stimulant and sedative. However, the preferred species for use are Cypripedium parviflorum and Cypripedium acaule, used as topical applications or tea.  
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