|Length||23.441 mi  (37.725 km)|
|West end||I-69 / US 41 in Henderson|
|East end||US 60 in Owensboro|
Named for John James Audubon, an early American naturalist, the Audubon's western terminus is at Interstate 69/ US 41; the eastern terminus is the US 60 bypass. The road opened on December 18, 1970 at a cost of $23.5 million and, at 23.4 miles (37.7 km), is the shortest of the nine roads in the state's parkway system. It is also the only road in the parkway system that has not had the name of a Kentucky politician attached to it.
The road carries the unsigned designation of Kentucky Route 9005 (AU 9005).
A white and gold shield was used along the Audubon Parkway until 2006, when a new, standardized blue-on-white marker was introduced for all of Kentucky's parkways.
The Audubon Parkway, as with all eight of the other parkways, was originally a toll road from its 1970 opening until 2006. The only toll plaza on the Audubon was located at the Exit 10 interchange at Kentucky Route 416 near Hebbardsville, in eastern Henderson County.  
The Audubon and the nearby William H. Natcher Parkway, which opened in 1972, were the last two remaining tolled parkways in Kentucky’s parkway system. They both had their tolls removed on Tuesday, November 21, 2006. Ernie Fletcher, who was governor at the time, announced the removal of the tolls at the Natcher Parkway's Hartford toll plaza in Ohio County on September 27, 2006. Fletcher himself manned the end loader which demolished one of the Audubon's Hebbardsville toll booths during a press conference and ceremony which heralded the end of toll collections.
With the end of toll collection, the Hebbardsville interchange was modified slightly, although its past as the parkway's only toll plaza remains in the modified-cloverleaf layout of the ramps, a trait shared by most such interchanges on Kentucky's other parkways. The islands where the toll booths were mounted were removed, and the pavement at the interchange was smoothed over, although the rumble strips approaching the interchange from both directions remain as of July 2009. During 2008, the former toll plaza office on the south side of the interchange was demolished
Daviess County officials first proposed in 2005 that the Audubon be upgraded to an Interstate highway, specifically suggesting the number Interstate 369. This would, according to the proposal, take place if and when Interstate 69 is extended southward from Indianapolis through Evansville, Indiana and across the Ohio River near Henderson, Kentucky. The I-369 "spur" would connect Owensboro, Kentucky to I-69.
The Audubon would have to be significantly upgraded in order for it to be approved as an Interstate highway; specifically, the shoulders would need to be widened and the median would have to either be widened or have a safety barrier constructed along its entire 24-mile (39 km) length.
The Audubon Parkway became a High Priority Interstate Corridor, referred to as the "Future Interstate Route 69 Spur" in the SAFETEA-LU Technical Corrections Act of 2008 which became Public Law 110-244 on June 6, 2008. New "Future I-69 Spur" Signs were placed on the parkway on September 2008.
|Henderson||Henderson||0.000||0.000||0||I-69 / US 41 – Henderson, Madisonville||Western terminus and signed as exits 0A (north) and 0B (south); I-69 exit 77|
|||5.389||8.673||5||KY 1078 – Zion||Constructed in 1986|
|Hebbardsville||10.175||16.375||10||KY 416 – Niagara, Hebbardsville||Former toll plaza; original exit|
|Daviess||||18.043||29.037||18||KY 1554 – Stanley, Sorgho||Constructed in 1986|
|Owensboro||23.441||37.725||24||US 60 to Natcher Parkway – Owensboro, Hawesville||Eastern terminus and signed as exits 24A (south) and 24B (north); US 60 Bypass exit 10|
|1.000 mi = 1.609 km; 1.000 km = 0.621 mi|
- "Kentucky Transportation Cabinet — Division of Planning — Highway Information System Official Milepoint Route Log Extract". Archived from the original on 30 April 2007. Retrieved 2007-04-08.
- Kentucky Department of Highways (1973). Kentucky Official Highway and Parkway Map (PDF) (Map). c. 1:760,320. Frankfort: Kentucky Department of Highways. Audubon Parkway toll schedule inset.
- Rand McNally (2002). "Kentucky" (Map). The Road Atlas (2002 ed.). 1 in≈30 mi. Chicago: Rand McNally. p. 42.