Draft:Amplicon Sequence Variant

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Amplicon Sequence Variant (ASV) is a term used to refer to individual DNA sequences after applying a denoising algorithm, such as DADA2.[1] ASVs are largely replacing the more traditional Operational Taxonomic Units (OTUs) generated by clustering to some similarity threshold with software such as QIIME.[2]

References[edit]

  1. ^ Holmes, Susan P.; Johnson, Amy Jo A.; Andrew W. Han; Rosen, Michael J.; Paul J. McMurdie; han, Benjamin J. Calla (July 2016). "DADA2: High-resolution sample inference from Illumina amplicon data". Nature Methods. 13 (7): 581–583. doi:10.1038/nmeth.3869. ISSN 1548-7105. PMC 4927377. PMID 27214047.
  2. ^ Kuczynski, Justin; Stombaugh, Jesse; Walters, William Anton; González, Antonio; Caporaso, J. Gregory; Knight, Rob (December 2011). "Using QIIME to analyze 16S rRNA gene sequences from Microbial Communities". Current protocols in bioinformatics / editoral board, Andreas D. Baxevanis ... [et al.] CHAPTER: Unit10.7. doi:10.1002/0471250953.bi1007s36. ISBN 978-0471250951. ISSN 1934-3396. PMC 3249058. PMID 22161565.